Fig. 2.
Fig. 2. A2AR are responsible for adenosine-induced accumulation of cAMP in murine thymocytes. / (A,B) Thymocytes from wild type (+/+) but not from A2AR gene deficient mice (−/−) respond to exposure to A2AR agonist CGS21680 by cAMP accumulation. Ex vivo thymocytes from wild type mice (A, +/+) or from A2AR deficient (B,−/−) mice were incubated 30 minutes with A2AR agonist CGS21680 in the presence of A2AR antagonist ZM241385, and cAMP accumulation was measured as described in “Materials and methods.” (C) Northern blot analysis of mRNA expression for A1, A2A, and A3receptors in mouse thymocytes (lane 1) and thymocyte cell line established from p53 −/− mice (lane 2). Samples in lane 3 represent mRNA from brain tissues (positive control for A1receptor mRNA) and the mastocytoma cell line P815 (positive control for A3 receptor mRNA).

A2AR are responsible for adenosine-induced accumulation of cAMP in murine thymocytes.

(A,B) Thymocytes from wild type (+/+) but not from A2AR gene deficient mice (−/−) respond to exposure to A2AR agonist CGS21680 by cAMP accumulation. Ex vivo thymocytes from wild type mice (A, +/+) or from A2AR deficient (B,−/−) mice were incubated 30 minutes with A2AR agonist CGS21680 in the presence of A2AR antagonist ZM241385, and cAMP accumulation was measured as described in “Materials and methods.” (C) Northern blot analysis of mRNA expression for A1, A2A, and A3receptors in mouse thymocytes (lane 1) and thymocyte cell line established from p53 −/− mice (lane 2). Samples in lane 3 represent mRNA from brain tissues (positive control for A1receptor mRNA) and the mastocytoma cell line P815 (positive control for A3 receptor mRNA).

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