Adenosine inhibits in vitro TCR-triggered activation of ADA-deficient T cells in the absence of ADA inhibitors.

Ex vivo spleen T cells from ADA −/− and ADA +/+ littermates were incubated in 96-well plates with immobilized anti-CD3 mAb (5 μg/mL mAb) or with serum-free, ADA-free media alone (0 μg/mL mAb) in the presence or absence of adenosine (100 μmol/L), and 16 hours later, the TCR-triggered up-regulation of T-cell activation markers CD69 in T cells was evaluated by flow cytometry.