Phenotypic analysis of cell surface antigens on allogeneic CTLs.

CTL effectors were generated as described in “Materials and methods.” (A) The CD4/CD8 ratio in the effector T-cell population was determined by double staining with CD8-PerCP and CD4-APC. (B) The presence of CD56⁺/CD16⁺ NK cells was analyzed by double staining with CD16-FITC and CD56-APC. Data are representative of cell surface staining from at least 3 separate experiments in which CTLs were generated.