Dose-dependent stimulatory effect of anti-CD11b/c mAbs and soluble CD23 chimeric proteins on the steady-state level of pro–IL-1β mRNA.

Nonadherent human monocytes (7 × 10⁶ cells) were untreated or incubated for 1 hour with various concentrations of the following effectors: A, anti-CD11b (clone 44), B, anti-CD11c (BU15), C, ZZ-CD23, D, MBP-CD23 and E, anti-CD11a (BU17, 5 μg/mL), ZZ-Eselectin (2 μg/mL), and ZZ-Pselectin (2 μg/mL). Then cells were harvested and RNA isolated and analyzed by Northern blot hybridization with pro–IL-1β and GAPDH cDNA probes as described in “Materials and methods.”