Fig. 3.
Time course of pro–IL-1β mRNA induction by β2 integrin engagement.
Monocytes (6-8 × 106 cells) were untreated or stimulated for various times with the following effectors: A, anti-CD11a (BU17, 2 μg/mL), B, anti-CD11b (44, 2 μg/mL), C, anti-CD11c (BU15, 2 μg/mL), D, ZZ-CD23 (1 μg/mL), and E, MBP-CD23 (1 μg/mL). RNA were isolated and analyzed for pro–IL-1β and GAPDH mRNAs expression as previously described. Results are representative of 3 distinct experiments.
