Fig. 6.
Analysis of pro–IL-1β mRNA stability in monocytes activated through CD11b or CD11c engagement.
Northern blot analysis of the decay of pro–IL-1β mRNA in human monocytes (7 × 106) stimulated with anti-CD11b (5 μg/mL, panel A), anti-CD11c (5 μg/mL, panel B), ZZ-CD23 (1 μg/mL, panel C), or MBP-CD23 (1 μg/mL, panel D). Cells were untreated (lane 1) or activated for 1 hour (lane 2) with the above effectors. Then DRB (60 μmol/L) was added and cells were incubated for a further 0.5, 1, 2, 4, and 6 hours (lanes 3 to 7, respectively). The level of ribosomal 18S RNA visualized by ethidium bromide was used as a control of the total RNA level. Right-hand side of the figure shows the densitometric scanning quantification of pro–IL-1β mRNA level.
