Fig. 9.
Time course of stimulation of the phosphorylation of p38/SAPK2 kinase in human monocytes activated by anti-CD11b mAb, anti-CD11c mAb, or sCD23 fusion proteins.
Monocytes (5 × 106 cells) were stimulated as depicted in the legend of Figure 7, ie, anti-CD11a (5 μg/mL), anti-CD11b (5 μg/mL), anti-CD11c (5 μg/mL), MBP-CD23 (1 μg/mL), and ZZ-CD23 (5 μg/mL) in panels A to E, respectively. Cell lysates were analyzed by Western blot using a specific antiphospho-p38 antibody. Western blots were stripped and reprobed with anti-p38 rabbit polyclonal antibody as a loading control. Results are the most representative of 4 distinct experiments.
