Ristocetin- and botrocetin-induced binding of rvWF-WT and type 2B rvWFs to platelets.

rvWF-WT (open bars) or mutated 2B rvWF-L697V (diagonal hatched bars), -R578Q (black bars), -V553M (gray bars), -M540MM (vertical hatched bars), and -V551F (horizontal hatched bars) labeled with¹²⁵I-MoAb 505 were incubated with paraformaldehyde-fixed platelets for 1 hour at room temperature in the presence of varying concentrations of ristocetin (0-1 mg/mL; panel A) or botrocetin (0-1 μg/mL; panel B). Results were expressed as outlined in the legend to Figure 4. Means ± SEM from 3 experiments were performed in duplicate.