Profiles of correlated cellular hemoglobin contents after treatment with 30% FCS or 10 ng/ mL rhTGFβ1.

Comparison between continuous exposure (days 0-7, left column) and exposure for the first 4 days of culture (days 0-4, right column). Analysis on day 7 of culture. Cultures were mixed into single-cell suspensions and the correlated contents of HbF and HbA for each individual cell measured by 2-color flow cytometry. Each analyzed cell sample results from a mixture of about 100 colonies. Each profile results from 10 000 intact nucleated cells with normal DNA content, gated by Hoechst fluorescence (see “Materials and methods”). The proportions of F+ cells (percentage of all Hb+ cells, see “Materials and methods”) are indicated in the upper right corner of each profile.