Fig. 2.
Fig. 2. Association of the βc chain and SHIP with Shc is mediated by interaction of NXXpY motifs and the PTB domain. / Lysates from untreated IC2 cells (−) or IC2 cells treated (+) with IL-3 (100 ng/mL) for 5 minutes were precipitated with the GST–PTB-domain fusion protein in the presence and absence of phosphopeptides corresponding to the NXXpY motifs of SHIP (p145-1 and p145-2) and the βc chain (pIL-3Rβ) or a control phosphopeptide (p145-3), at the indicated concentrations. The precipitates were subjected to Western blot analysis with anti-pTyr antibodies (A). (B, C) Peptide competition with phosphopeptides pIL-3Rβ and control p145-3 was reprobed with anti-SHIP or anti-βc antibodies. Similar results were obtained with p145-1 and p145-2 phosphopeptides (data not shown).

Association of the βc chain and SHIP with Shc is mediated by interaction of NXXpY motifs and the PTB domain.

Lysates from untreated IC2 cells (−) or IC2 cells treated (+) with IL-3 (100 ng/mL) for 5 minutes were precipitated with the GST–PTB-domain fusion protein in the presence and absence of phosphopeptides corresponding to the NXXpY motifs of SHIP (p145-1 and p145-2) and the βc chain (pIL-3Rβ) or a control phosphopeptide (p145-3), at the indicated concentrations. The precipitates were subjected to Western blot analysis with anti-pTyr antibodies (A). (B, C) Peptide competition with phosphopeptides pIL-3Rβ and control p145-3 was reprobed with anti-SHIP or anti-βc antibodies. Similar results were obtained with p145-1 and p145-2 phosphopeptides (data not shown).

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