Fig. 4.
The PTB domain, Y239 and Y317 of Shc are required for SHIP and Grb2 association.
Factor-deprived IC2 stable transfectants (4 × 107cells/sample) were stimulated with 100 ng/mL IL-3 for 5 minutes. Cells were lysed and immunoprecipitated with anti-FLAG antibodies. Protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and subjected to Western blot analysis with anti-pTyr, anti-SHIP, anti-Grb2, or anti-Shc antibodies.
