In vitro chemotaxis of HMECs and HUVECs toward MCP-1 is inhibited by anti-MCP-1.

(A) The migration of HMECs and HUVECs toward different concentrations of MCP-1 was quantitated as the number of cells per 10× field as described in the “Materials and methods” section. (B) Inhibition of the chemotactic responses of HMECs and HUVECs toward MCP-1 by the mAb 279. MCP-1 was used at the dose that induced maximal chemotactic responses (1 ng/mL). The migration toward medium alone (basal migration) was subtracted. *P < .001; **P < .025. One representative experiment is shown. The assay was repeated 5 times.