Rat aortic ring assay.

Rat aortic ring capillary sprouting in response to MCP-1 (1 ng/mL). Capillary sprouting occurred from the edge of the ring. (A) Negative control, (B) MCP-1 at 0.5 ng/mL, (C) MCP-1 at 5 ng/mL, (D) MCP-1 at 50 ng/mL, (E) MCP-1 at 500 ng/mL, and (F) EGF at 100 ng/mL was used as the positive control; 4× magnification. Note that MCP-1-induced endothelial cell sprout in a dose-responsive manner. One representative of 2 experiments is shown.