Blocking of MCP-1 inhibited lung tumor metastases of SCID mice bearing MDA-231 human tumor breast cancer cells.
SCID mice were injected intravenously with 20 μL of antiserum to ASGM1 on day 0, and 3 × 105 MDA231 human breast carcinoma cells were injected intravenously on day 1. Antibodies, including rabbit IgG (panel A) and 279 Ab (Panel B) at 25 μg/mouse (1 mg/kg), were given intraperitoneally to the mice on days 4, 8, 12, 16, 20, 24, and 28. For experimental metastasis experiments, mice from both groups were sacrificed on the 35th day after intravenous injection of the tumor cells. Lungs were extracted and fixed in formalin. Histological sections were stained with hematoxylin and eosin to evaluate tumor metastases. Photographs were taken at 150× magnification. The arrows indicate metastatic lesions. One representative field from 2 experiments is shown.
