Anti-IAP/CD47 mAb (B6H12) does not change the avidity/affinity of 4β1 (VLA-4) in BALL cells.

(A) Adhesion assay using the crystal violet method was performed as described in “Materials and methods.” B6H12 did not affect the adhesion to FN, whereas 8A2, an activating mAb against β1 integrin, significantly promoted the adhesion to FN. (B) The binding of FN to BALL cells was performed as described in “Materials and methods.” B6H12 had no effect on the FN binding to BALL cells. Data are mean ± SD of 3 independent experiments. Statistically significant differences from control values are indicated by *P < .01.