AdGFP transduction of CD38 ± subsets of mPB CD34⁺ cells.

(A) The purity of CD34⁺/CD38⁺ and CD34⁺/CD38⁻ selected cells was analyzed by dual color FACs immediately after separation with the use of magnetic beads. (A) Total cells stained with FITC- and PE-labeled isotype control antibodies. (B) Total cells stained with PE-conjugated anti-CD34 and FITC-conjugated anti-CD38 antibodies. (C) CD38⁻ subset following negative bead selection, stained with anti-CD34 and FITC-conjugated anti-CD38 antibodies. (D) Transduction of CD34⁺/CD38⁺ (□) and CD34⁺/CD38⁻(○) cells as assessed by FACs analysis 3 days after infection with AdGFP.