Flow cytometric sorting of transduced progenitor cells.

CD34⁺ mPB cells were transduced with AdGFP at an MOI of 2000. (A) An example of sorting gates set to collect GFP⁻ and total GFP⁺ cells as well as GFP⁺ cells that exhibited high, medium, and low fluorescence. Post-sort FACS analysis was performed to check the purity of the sort. (B) Mock-infected cells. (C) GFP⁺sorted cells. (D) GFP⁻ sorted cells.