CFU-Cs in sorted AdGFP-infected cells.

After sorting for GFP expression, colony assays were performed to assess CFU-C content of the fractionated cells. (A) Values represent the total number of CFU-Cs (calculated as frequency of CFU-Cs × the percentage of cells in each fraction) in GFP⁺ and GFP⁻ fractions relative to the total CFU-Cs in the control (AdGFP) population. Error bars indicate SEM from 6 independent experiments. (B) Total CFU-Cs in GFP⁺ fractions exhibiting low, medium, and high fluorescence in experiment 1. Values were calculated as the CFU-C frequency × the percentage of cells in each fraction. CFU-C frequency is an average calculated from triplicate plates. Error bars represent the SEM.