Surface phenotype of unilineage hematopoietic cells at day 14 of culture.

At day 14, cells obtained in unilineage cultures with the indicated cytokines were phenotypically characterized by double staining with anti-CD71 + anti-glycophorin A (erythroid antigens), anti-CD61 + anti-CD42b (megakaryocytic antigens), anti-CD15 + anti-CD11b (granulocytic antigens), or anti-CD16 + anti-CD14 (monocytic antigens). Percentages of cells in the respective quadrants are indicated. CD14⁺ cells were 65%. Quadrants were set based on negative controls treated with isotype-matched irrelevant mAbs (not shown).