Fig. 8.
Fig. 8. TRAIL-induced inhibition on pre-stimulated clonogenic erythroid progenitors (BFU-E) in semisolid cultures. / (A) Erythroid (BFU-E), megakaryocytic (CFU-meg), and granulocytic-macrophagic (CFU-GM) colonies were scored after 14 days of semisolid cultures. CD34+ cells were either seeded in semisolid cultures immediately after purification (white columns) or pre-stimulated in liquid cultures with specific cytokine cocktails for 2 days (black columns). Before seeding, cells were treated for 20 hours with irrelevant IgM mAb and rHis6-tag peptide (CONT), TRAIL, or anti-CD95 (CH 11) IgM mAb. Data are expressed as means ± SD of 4 separate experiments performed in duplicate. (B) Representative BFU-E are shown to document the difference in terms of colony size between TRAIL, CH11, and control cultures. Original magnification (400×).

TRAIL-induced inhibition on pre-stimulated clonogenic erythroid progenitors (BFU-E) in semisolid cultures.

(A) Erythroid (BFU-E), megakaryocytic (CFU-meg), and granulocytic-macrophagic (CFU-GM) colonies were scored after 14 days of semisolid cultures. CD34+ cells were either seeded in semisolid cultures immediately after purification (white columns) or pre-stimulated in liquid cultures with specific cytokine cocktails for 2 days (black columns). Before seeding, cells were treated for 20 hours with irrelevant IgM mAb and rHis6-tag peptide (CONT), TRAIL, or anti-CD95 (CH 11) IgM mAb. Data are expressed as means ± SD of 4 separate experiments performed in duplicate. (B) Representative BFU-E are shown to document the difference in terms of colony size between TRAIL, CH11, and control cultures. Original magnification (400×).

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