Fig. 1.
Fig. 1. Bcl-2 expression in HL-60 and HL-60-DOX cells. / (A) Flow cytometry shows that HL-60-DOX cells have a significantly higher Bcl-2 expression than HL-60 cells (P = .02). (B) HL-60-DOX and HL-60 cell lysates were Western blotted and probed with Bcl-2 and Bcl-XL polyclonal antibodies. Loading of lanes was controlled with actin, demonstrating that equivalent amounts of protein were blotted (results not shown). Antimouse peroxidase and enhanced chemiluminiscence were used to detect immunoreactive bands.

Bcl-2 expression in HL-60 and HL-60-DOX cells.

(A) Flow cytometry shows that HL-60-DOX cells have a significantly higher Bcl-2 expression than HL-60 cells (P = .02). (B) HL-60-DOX and HL-60 cell lysates were Western blotted and probed with Bcl-2 and Bcl-XL polyclonal antibodies. Loading of lanes was controlled with actin, demonstrating that equivalent amounts of protein were blotted (results not shown). Antimouse peroxidase and enhanced chemiluminiscence were used to detect immunoreactive bands.

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