Effect of Bcl-2-AS on Bcl-2 expression of HL-60 and HL-60-DOX cells.
Bcl-2 protein expression of HL-60 (A) and HL-60-DOX (B) was analyzed by flow cytometry as described above after gating on live cells. The relative channel number was measured from the upper limit of the negative control. The relative channel number of a series of calibrated FITC microbeads having levels of fluorescence intensity ranging from 8.857 × 103 to 206.086 × 103antibody-binding capacity (ABC) of equivalent soluble fluorochrome per bead was calculated (FCSC Quantum; Becton Dickinson) and a standard curve constructed. The relative channel number and the antibody-binding capacity per cell for the test samples were calculated using this standard curve. Results demonstrate lower Bcl-2 expression of cells treated with 8 μmol/L of Bcl-2-AS for 5 days.
