American Society of Hematology

Fig. 2.

$Fig. 2. Induction of cell death by chemotherapy drugs in the presence and absence of H2O2. / JLP 119 cells were treated with either VP-16, doxorubicin, cisplatin, or AraC followed 30 minutes later by the addition of the indicated concentrations of H2O2. After 22 hours of incubation, cells were harvested and analyzed by fluorescence microscopy, as described in “Materials and methods.” The fraction of total cells representing each form of cell death is shown. For ease of interpretation, the numbers of blue (membrane-intact) and red (PI-permeable) apoptotic cells were added together (□), as were the numbers of pyknotic/necrotic and necrotic cells (▪). The number of typical necrotic cells generally represented less than 10% of the number of pyknotic/necrotic cells. Each experiment was repeated at least 3 times. Error bars show the SD for the total number of dead cells.$

Induction of cell death by chemotherapy drugs in the presence and absence of H₂O₂.

JLP 119 cells were treated with either VP-16, doxorubicin, cisplatin, or AraC followed 30 minutes later by the addition of the indicated concentrations of H₂O₂. After 22 hours of incubation, cells were harvested and analyzed by fluorescence microscopy, as described in “Materials and methods.” The fraction of total cells representing each form of cell death is shown. For ease of interpretation, the numbers of blue (membrane-intact) and red (PI-permeable) apoptotic cells were added together (□), as were the numbers of pyknotic/necrotic and necrotic cells (▪). The number of typical necrotic cells generally represented less than 10% of the number of pyknotic/necrotic cells. Each experiment was repeated at least 3 times. Error bars show the SD for the total number of dead cells.

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