BPI induces HUVECs to detach from the matrix.

(A) Control HUVECs after 24 hours, (B) HUVECs cultured with 1.8 μmol/L BPI for 24 hours, (C) control HUVECs after 72 hours of culture, (D) HUVECs cultured with 1.8 μmol/L BPI for 72 hours. After 24, 48, and 72 hours, HUVECs were cultured under the indicated conditions and analyzed under an inverted microscope for the ratio of detached cells. The mean results of 3 independent experiments are shown. For clarity, error bars have been left out; variation was < 20% in all cases (E).