Fig. 7.
Fig. 7. TCRβ gene rearrangement in TEL-JAK2 leukemic cells. / (A) DNA was extracted from the thymus (T) from founder mouse 65, from 4 F1 animals of transgenic line 71 (71-11, 71-24, 71-10, and 71-4), and from a control thymus. Lymph nodes (LN) from transgenic mice 30 and 71-4 and the liver (L) from mouse 65 were also analyzed. DNA were digested by HindIII and analyzed by Southern blot using a jβ2-specific probe. An arrow points to germline configuration. TCRβ-rearranged bands (indicated by stars) are prominant in all leukemic samples. (B) DNA was extracted from a control thymus (lane 1), from the thymus of a diseased transgenic mouse 71-18 (lane 2), from nude mice tumors obtained following transplantation either of the leukemic cells of animal 71-18 (Nu18, lane 3) or following secondary transplantation of Nu18 tumor cells (lanes 4 and 5), from nude mice tumors obtained from the transplantation of sorted SP (lanes 6-8) or DP cells (lane 11), and from the sorted CD8+SP and DP populations derived from transplanted tumors Nu18/SP4 and Nu18/DP1 (lanes 9-10 and 12-13) (see also Table 3). DNA were digested by HindIII and analyzed by Southern blot using a jβ2-specific probe. Germline configuration is indicated by an arrow. TCRβ-rearranged bands (indicated by stars) are prominant in all leukemic samples. (C) DNA was extracted from a control thymus (lane 1), from the thymus of diseased transgenic mouse 71-24 (lane 2), from nude mice tumors obtained either following transplantation of the leukemic cells of animal 71-24 (Nu24, lane 3) or following secondary transplantation of Nu24 tumor cells (Nu24/1, lane 4), from nude mice tumors obtained from the transplantation of sorted SP (Nu24/SP1, lane 7), and from the sorted DP and CD8+ SP populations derived from either transplanted tumors Nu24/1 (lanes 5 and 6) or Nu24/SP1 (lanes 8 and 9) (see also Table 3). DNA were digested byHindIII and analyzed by Southern blot using a jβ2-specific probe. Germline configuration is indicated by an arrow. TCRβ-rearranged bands (indicated by stars) are prominant in all leukemic samples.

TCRβ gene rearrangement in TEL-JAK2 leukemic cells.

(A) DNA was extracted from the thymus (T) from founder mouse 65, from 4 F1 animals of transgenic line 71 (71-11, 71-24, 71-10, and 71-4), and from a control thymus. Lymph nodes (LN) from transgenic mice 30 and 71-4 and the liver (L) from mouse 65 were also analyzed. DNA were digested by HindIII and analyzed by Southern blot using a jβ2-specific probe. An arrow points to germline configuration. TCRβ-rearranged bands (indicated by stars) are prominant in all leukemic samples. (B) DNA was extracted from a control thymus (lane 1), from the thymus of a diseased transgenic mouse 71-18 (lane 2), from nude mice tumors obtained following transplantation either of the leukemic cells of animal 71-18 (Nu18, lane 3) or following secondary transplantation of Nu18 tumor cells (lanes 4 and 5), from nude mice tumors obtained from the transplantation of sorted SP (lanes 6-8) or DP cells (lane 11), and from the sorted CD8+SP and DP populations derived from transplanted tumors Nu18/SP4 and Nu18/DP1 (lanes 9-10 and 12-13) (see also Table 3). DNA were digested by HindIII and analyzed by Southern blot using a jβ2-specific probe. Germline configuration is indicated by an arrow. TCRβ-rearranged bands (indicated by stars) are prominant in all leukemic samples. (C) DNA was extracted from a control thymus (lane 1), from the thymus of diseased transgenic mouse 71-24 (lane 2), from nude mice tumors obtained either following transplantation of the leukemic cells of animal 71-24 (Nu24, lane 3) or following secondary transplantation of Nu24 tumor cells (Nu24/1, lane 4), from nude mice tumors obtained from the transplantation of sorted SP (Nu24/SP1, lane 7), and from the sorted DP and CD8+ SP populations derived from either transplanted tumors Nu24/1 (lanes 5 and 6) or Nu24/SP1 (lanes 8 and 9) (see also Table 3). DNA were digested byHindIII and analyzed by Southern blot using a jβ2-specific probe. Germline configuration is indicated by an arrow. TCRβ-rearranged bands (indicated by stars) are prominant in all leukemic samples.

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