Fig. 1.
Limiting dilution experiments for rearranged IGH and TCRD.
Rearranged IGH and TCRD amplified segments are shown by ethidium bromide gel staining. (A) IGH, leukemic cell DNA. (B) TCRD, leukemic cell DNA. (C) IGH, Guthrie card DNA. (D) TCRD, Guthrie card DNA. Standard limiting dilution (10−1 through 10−6) was prepared with positive control cells. The limit for detecting the IGH rearrangement was approximately 10−3 compared with 10−4 for TCRD in leukemic cells DNA. M indicates DNA size marker; P, amplified product from 100 ng positive control DNA; and N, amplified product from negative control DNA.
