PCR analysis for rearranged IGH and TCRD.

Amplification of rearranged IGH and TCRD from 7 cases in our study. Comparison of the PCR products from cases 1, 2, and 3 showed no size differences between Guthrie card and leukemic cell DNAs representing the IGH. We could not detect the rearranged TCRD from leukemic cell DNA and corresponding Guthrie card DNA in case 2. For case 4, the rearranged IGH product from Guthrie card DNA consisted of 2 different-sized fragments (4a and 4b), 1 of which (4a) was identical to the fragment amplified from leukemic cell DNA. Sequencing of the (a) and (b) DNAs revealed the same V-D-J or V-D rearrangements (Tables 3and 4). M indicates DNA size marker; G, PCR products from Guthrie card DNA; and BM, PCR products from leukemic bone marrow cell DNA.