Fig. 2.
Electrophoretic mobility shift assay of AML samples.
AML cells (patients 17, 18, 19) were either unstimulated or stimulated with 10 ng/mL IL-6 or IFN-γ as indicated. Nuclear extracts were isolated and analyzed for Stat3 binding activity by electrophoretic mobility shift assay using a 32P-labeled IRE probe from the human ICAM promoter. Supershift assays were performed using antibodies against Stat1 and Stat3, and in competition experiments a 100-fold molar excess of IRE or an aspecific probe was used.
