HHV-7 expression determined by specific RT-PCR in liquid cultures.

RNA was extracted from uninfected (mock) and HHV-7–infected cultures at day 7 of culture. For RT-PCR, equivalent amounts (200 ng) of RNA samples before (−) and after (+) RT were used as template for the amplification reaction with the HV7 and HV8 primers (U10 ORF). Control indicates positive control (RNA from HHV-7–infected SupT1 cells) showing the expected amplification products of 186 bp; lane M, 100-bp ladder of molecular weight marker; blank lanes are samples without RNA. The data are representative of 3 experiments from separate infections.