RT-PCR analysis of the transcripts 50 and 134 from CD34⁺ cells.

mRNA from enriched CD34⁺ cells either nonstimulated (0) or stimulated (4 or 8 hours) with GM-CSF was RT. cDNA from each RT reaction was PCR-amplified using specific primers for the detection of a 445 bp in the RBM3 transcript or a 567 bp in the NACAtranscript sequences, together with the amplification of the β2 microglobulin transcript (268 bp). The products were electrophoresed on a 2% agarose gel, blotted, and hybridized with oligonucleotides within the RBM3, NACA, and β2 microglobulin (β2 m) respective amplified PCR region.