Comparison of mouse, rat, and human amino acid sequences.

Alignment of amino acid sequences of mouse, rat, and human β-adducin (β-Add97 form). The putative Δ9-13 β-adducin gene product generated from β-adducin −/− mice is indicated as “mouse −/−.” Filled triangles and numbering indicate the represented exons according to Gilligan et al.7 White triangles in exons 10 and 11 indicate the end of the β-adducin head domain (aa 1-338) and the beginning of the tail region (aa 419-726), respectively. The neck region (aa 339-418) is located in between the white triangles. The boxes indicate the different functional domains: oligomerization domain (aa 335-436, 99% identity),15calmodulin-binding domain (aa 425-461, 97.2% identity),27PKA phosphorylation region (aa 523-534, 100% identity),13and calmodulin-binding domain (MARCKS domain, aa 705-721, 100% identity).2 12 Asterisks indicate identical residues, whereas 2 dots indicate conservative substitutions. Aa sequences have an overall 90.0% identity and 94.8% similarity.