Osmotic fragility test of RBCs.

Erythrocytes from +/+ (⋄), +/− (□), and −/− (▵) mice were subjected to an OF test as described in the “Materials and methods” section. Results (mean ± standard error of 5 8- to 10-month-old animals/group) are expressed as percentage of lysis in graded salt concentrations. C₅₀ values were determined by logarithmic linearization of the OF curve.26