Cotransfection of LF89, mutant LF89, and an expression C/EBP plasmid (pMSVC/EBP) in 32Dwt18 cells.

32Dwt18 cells were transiently cotransfected with 10 μg of LF89 or a C/EBP mutant of the LF89 plasmid and increasing amounts (0-10 μg) of an expression plasmid for C/EBPα (pMSVC/EBPα). Two micrograms of pCMVβgal plasmid was included in each transfection. Cells were harvested 24 hours after transfection and reporter gene activity was measured. Normalized luciferase values are represented as a ratio of enzyme activity of LF89 plus pMSVC/EBPα to that of LF89 without pMSVC/EBPα. The mean ± SE value for 3 experiments performed in duplicate is represented.