Transient cotransfection of mutant LF Sp1 plasmids with a C/EBP expression plasmid in 32Dwt18 cells.

32Dwt18 cells were transiently cotransfected with 10 μg of LF89, SP1A, and SP1B mutant forms of the LF89 plasmid, with and without 10 μg of an expression plasmid for C/EBPα (pMSVC/EBPα); 2 μg of pCMVβgal plasmid was included in each transfection. Cells were harvested 24 hours after transfection and reporter gene activity was measured. Normalized luciferase values from 1 of 3 experiments performed in duplicate are represented.