Transient cotransfection analysis of LF promoter plasmids with an SP1 expression plasmid in Drosophila S2 cells.

Drosophila S2 cells (Sp1 negative) were transiently cotransfected with LF89 and Sp1A, Sp1B, and C/EBP mutant forms of the LF89 plasmid, with and without 5 μg of an expression plasmid for Sp1 (pPACSp1). Cells were harvested 48 hours after transfection and reporter gene activity was assessed. Luciferase values were normalized as per microgram of total protein. One representative experiment of 3 performed in duplicate is shown.