Fig. 1.
TheμLCRβprRlucγprFlucconstruct.
It contains a 3.1-kb μLCR cassette linked to a 315-bp human β-globin gene promoter driving the renilla luciferase gene and a 1.4-kb Aγ promoter driving the firefly luciferase gene. S, SnaBI (Genebank, HUMHBB 61869); N, NcoI (Genebank, HUMHBB 62185); H, HindIII (Genebank, HUMBB 388057); A,AhaII (Genebank, HUMHBB 39461). ▪ represent the 130-bp SV40-polyadenylation site.