Dose-response relationships for 2-, 3-, and 4-hydroxylbutyrate.

(A, B) Results from experiments with low and high concentrations of the compounds, respectively, are shown. For each compound, experiments were performed in triplicate for each concentration shown, and means are plotted. ▪ indicates dose-response relationship for control experiment with sodium butyrate. Experiments were performed in triplicate at each concentration, and means and standard deviations are plotted. Concentrations greater than 2 mmol/L were toxic to the cells.