In vitro transcription and translation assay.

The 631delT RAG1 gene and the wt RAG1 gene were both cloned in a pGEM-T easy expression vector using the T7 promotor. Transcription and translation of the 631delT RAG1 gene showed absence of the 119-kd wt protein band, which was present in the lane of the wt RAG1 gene. The 631delT showed only the smaller 100-kd N-terminal truncated protein band, which was also present in the wtRAG1 gene lane.