Western blotting after transfection of COS cells with wt RAG1 and 631delT RAG1 constructs.

RAG1 proteins with c-myc tag were detected using a c-mycantibody and were visualized by enhanced chemiluminescence. The upper band in the wt lane represents the 119-kd wt RAG1 protein, which is absent in the 631delT RAG1 lane. Both the wt RAG1 and the 631delT RAG1 gene express the 100-kd N-terminal truncated protein. In both lanes additional protein products are seen, representing degradation products of the RAG1 protein, which is in line with the short half-life of this protein.