Inhibiting the transfer of TF cell fragments to platelets.

Platelets were activated on collagen, and (A) platelet-particle hybrids were double stained. TF was visualized by rabbit pAbs against hTF followed by goat antirabbit antibodies conjugated to PE, whereas platelets were stained with an FITC-conjugated mAb directed against GPIIb. Plots in panels B, C, and D show the GPIIb⁺ particles (platelets). (B) TF was found on 14% of platelets. (C) A blocking anti-CD15 antibody inhibited the attachment of TF-containing particles to platelets. (D) The anti-TF mAb (hTF-1) also reduced TF adherence to platelets.