HGF-induced matrigel invasion of isolated monocytes.

Primary cultured monocytes migrate into matrigel, pass through a porous membrane filter, and adhere to the bottom side, where they are fixed and counterstained. (A) Several cells may simultaneously pass through a single pore (400 ×  magnification of the bottom side of a polycarbonate membrane filter). Invasions of unstimulated (B) and HGF-stimulated monocytes with diffuse (C) and irregular (D) cell distribution after 18 hours of incubation are shown (100-fold magnification of the bottom side of the membrane).