Extent and time course of induced monocytic matrigel invasion.

(A) Invasion of growth factor–depleted matrigel by monocytes derived from 6 different healthy donors, after incubation with HGF and without HGF (control) for 18 hours and 48 hours (separate representation for each donor). For relative comparisons of samples from the different donors, the invasion index of all HGF stimulations at 18 hours was arbitrarily set at 1. (B) Cumulative analysis of the monocytic invasion assays shown in Figure 4A. Each symbol represents a single experiment. The respective medians are indicated by a horizontal line. (C) Matrigel invasion of monocytes from 2 different donors, after incubation with HGF and without HGF (control). Specificity of the HGF-mediated biologic effect was controlled by preincubation of the HGF with a specific anti-human HGF neutralizing antibody for 30 minutes. The control incubation value for each donor sample was arbitrarily set at 1.