Fig. 1.
RNA and genomic analysis of human EPR-1/survivin.
(A) Hybridization of multiple tissue Northern blots (Clontech) withEPR-1 or survivin RNA-specific riboprobes. Probe A is complementary to the first 226 nucleotides of EPR-1 plus the first 94 nucleotides of the intervening sequence. Probe B is complementary to nucleotides 664 to 972 of EPR-1 (GenBank accession number L26245). (B) PCR amplification of survivingenomic DNA sequences from human genomic DNA (upper panel) or of survivin cDNA sequences (lower panel) with 4 different primer sets (see “Materials and methods”). Primer combinations used for the reactions are as follows: lane 1: a-c; lane 2: a-d; lane 3: b-c; lane 4: b-d.