Invasion of THS119 cells into the TBR59 cell layers.
The 2.5 × 106 THS119 cells were cultured on TBR59 cells for 3 hours at 33°C with or without supplements. Adhered and suspended THS119 cells were retrieved by incubation and rinsed with EDTA-PBS. Invading cells remained under the stromal cell layer, and cells staying on the surface of the stromal cells were retrieved and counted. Invaded cell numbers were obtained by subtracting the numbers of noninvaded cells from the input cell numbers. Hi-FBS, heat-inactivated FBS at 90°C for 10 minutes; De-FBS, FBS delipidated by charcoal stripping; AlbMax, lipid-conjugated BSA. Data are the mean ± SD of 3 dishes: 5% FBS, AlbMax, and Hi-FBS versus 0% FBS (P < .0001 by Bonferroni/Dunn test); 0% FBS versus 1% BSA (P = .1639 by Bonferroni/Dunn test); and 0% FBS versus De-FBS (P = .028 by Bonferroni/Dunn test).
