Fig. 3.
Effect of several kinds of lipids on the ability of THS119 to invade TBR59 cell layers.
The 2.5 × 106 THS119 cells were cultured on TBR59 cell layers for 3 hours at 33°C with or without 5 μg/mL of indicated lipids. Adhered and suspended THS119 cells were retrieved by incubation and rinsed with EDTA-PBS. Invaded cell numbers were obtained by subtracting the numbers of noninvaded cells from the input cell numbers. Sph, sphingosine; S1P, sphingosine-1-phosphate; Cra, ceramide; C1P, ceramide-1-phosphate; LPA, lysophosphatidic acid; LPC, lysophosphatidyl choline; LPS, lysophosphatidyl serine; and SM, sphingomyelin. Data are the mean ± SD of 3 dishes: S1P, and LPA versus 0% FBS (P < .0001 by Bonferroni/Dunn test).
