Effect of PTx and C3 exotoxin on the ability of THS119 cells to invade TBR59 cell layers.
SLO permeabilized 2.5 × 106 THS119 cells were cultured on TBR59 cell layers for 3 hours at 33°C. (A) 200 ng/mL PTx or 10 μg/mL C3 exotoxin (C3) was added during permeabilization. Uptake of large molecules during permeabilization as measured by uptake of FITC-Dextran (0.5 mg/mL) was seen in 46% of the total cells. Cell viability was monitored after permeabilization by addition of 200 ng/mL propidium iodide, and was 88%. (B) Dose-response relation for toxins. PTx was added at 100 to 800 ng/mL and C3 exotoxin was added at 5 to 40 μg/mL during permeabilization. Uptake of large molecules was seen in 49% of total cells and cell viability was 84%. Data are the mean ± SD of 3 dishes: C3, and PTx versus 5% FBS (P < .0001 by Bonferroni/Dunn test) in (A); C3, and PTx versus 5% FBS (P < .0001 by Bonferroni/Dunn test) in (B), except for 5 μg/mL C3 versus 5% FBS (P = .0004 by Bonferroni/Dunn test).
