Fig. 5.
Expression of edg-1 and edg-2 by parental THS119 cells and cytokine-dependent THS119 sublines.
Total RNA (0.5 μg) from bone marrow, THS119, stroma-independent IL-3–dependent THS119 subline (THS119IL-3), and stroma-independent IL-7–dependent THS119 subline (THS119IL-7) were analyzed by RT-PCR.edg-1, 442 base pairs (bp) fragment specific for edg-1expression; edg-2, 1139 bp specific fragment for edg-2expression; HPRT, 516 bp specific fragment for HPRT expression.