Expression of edg-1 and edg-2 in long-term bone marrow culture cells.

Cells were obtained from the culture medium (floating hematopoietic cells), or were collected after enzyme treatment, followed by further short-term incubation to separate the stromal cells from the hematopoietic cells in cobblestone areas. Total RNA (0.5 μg) was analyzed by RT-PCR. edg-1, 442 bp fragment specific foredg-1 expression; edg-2, 1139 bp specific fragment foredg-2 expression (arrow); HPRT, 516 bp specific fragment for HPRT expression.