Fig. 2.
Exogenous NO induces apoptosis in bone marrow macrophages.
(A) NO production by SNAP; 105 macrophages were treated with 50 μmol/L of SNAP for the indicated times and the production of NO was determined. (B) Time course of SNAP-induced apoptosis; 105 macrophages were treated with 50 μmol/L SNAP at the indicated times. Apoptosis was measured by ELISA. (C) The cells were treated for 24 hours with the indicated concentrations of SNAP, and apoptosis was detected as indicated above. Each experiment was performed in triplicate and the results of 1 representative of 2 independent experiments are represented as the mean value ± SD.
