Fig. 3.
LPS induces the expression of iNOS and the production of NO.
The expression of iNOS induced by LPS was measured by Northern blot (A) or Western blot (B) as described in the “Materials and methods” section. (C) LPS induces the production of NO; 106 BMDM cultured in media without phenol-red were stimulated with 100 ng/mL LPS, and the supernatants were harvested at the indicated times. NO production was measured as the nitrite/nitrate levels. Each point was performed in triplicate and represented as the mean ± SD. These figures are representative of 3 independent experiments.
